Sceyence Fear

1. Question
Why does the mouse with the mutated ACTM gene in T cells go blind? How can we prevent and cure the blindness?

2. Key words:
mouse
gene mutation
blindness
T cells
ACTM gene
abnormal eye structure
eye infection
medical treatment
human eye diseases

3. Relevant questions:
A mouse has many genes so why was the ACTM gene mutated?
Why should the blindness in mice be investigated?

How can we dissect and check the eyes of the mouse?
How can we prevent and cure blindness of mice or humans?

What is the structure of the normal eyes of mice and humans?
What are the possible reasons leading to blindness in mice and humans?

Where is the ACTM protein localized in T cells?
Where do T cells stay in the eyes of mice or humans?

When do the mutated mice go blind?
At what stage should mice with eye diseases be treated to prevent and/or cure blindness?

Who, in addition to people with serious diabetes, go blind?
Who among blind humans have, or may have, this mutated gene?

4. Three sources:
(1). Q liu and K. siminovitch, unpublished data
(2). Mallick, I (2007, August 13). T-cells - What are T-Cells?. About.com Leukemia and Lymphoma. Retrieved November 3, 2012, from http://lymphoma.about.com/od/glossary/g/tcells.htm
(3). EYE STRUCTURE AND FUNCTION. (n.d.) My Eye World. Retrieved November 3, 2012, from http://www.myeyeworld.com/files/eye_structure.htm

5. 3 equations:
(1). frequency of blind mice = (number of blind mice / total number of mice) x 100%
(2). frequency of T cells in an eye = (number of T cell in an eye / total number of cells in the eye) x 100%
(3). frequency of another kind of cell in an eye (B Cells) = (number of another kind of cell (B Cells) / total number of cells in an eye) x 100%

Control:
Wild Type Mouse without a mutated ACTM gene

Independent:
Mice with mutated ACTM gene

Dependent:
Blindness